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Fig. 8. Evidence for increased apoptosis and reduced proliferation in conditional mutant lungs. (A-F) Sections of cre(-) and cre(+) lungs at E14.5, E15.5 and E18.5. Note the presence of cellular debris (arrowheads), and the irregular shape and smaller size of the epithelial cells in the conditional mutant lungs (D-F). (G-L) Immunofluorescence for cleaved caspase 3 and DAPI (nuclei). Note abundant apoptotic cells in the lumen, epithelium and mesenchyme of cre(+);Nmyc^flox/+ and cre(+);Nmyc^flox/flox lungs at E15.5, E16.5 and E18.5 (arrowheads). The faint fluorescence seen in J is from red blood cells. (M-O) BrdU labeling. Most distal epithelial cells in the E15.5 wild-type lung are positive for BrdU after a 1-hour pulse. By contrast, note the reduced epithelial labeling of mutant lungs at E15.5 and E16.5 (arrowheads). Scale bars: 50 µm.

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