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Fig. 9. Differentiation of epithelial cells in conditional mutant lungs. Double in situ hybridization for Sftpc and Scgb1a1 in cre(-);Nmycflox/+ (A) and cre(+);Nmycflox/flox (B) lungs at E18.5. Note the paucity of type II cells in the mutant lung. (C,D) Immunofluorescence for Gp38 expressed on the apical surface of type I cells at this stage. Note the presence of attenuated presumptive type I cells lining the large sacs. (E) RT-PCR for Aqp5, SftpA and actin. Expression of Aqp5 is prematurely increased in cre(+);Nmycflox/flox lung at E16.5. Scale bars: 50 µm.





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