Supplemental Figure 1
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Fig. S1. Costal2
is both necessary and sufficient for the repression of dpp and ptc, two Hedgehog target genes in the wing imaginal
disc. lacZ reporter proteins
produced by both dpp-lacZ and ptc-lacZ are nuclear because of a nuclear localization signal
incorporated in the coding region. (A) The expression of dpp-lacZ, as shown by nuclear bGal staining (red), is in a wide swath of nuclei, 12- to
15-cell-diameters wide, in anterior cells at the AP boundary. En protein is
shown in blue. (B-C) mRNA in situ using antisense dpp probe in a wild-type (B) and mutant (C) disc in
which cos2–
clones have been generated. (D,E) Overexpression of cos2 is sufficient to repress dpp transcription in a cell-autonomous manner. (D) dpp-lacZ expression (red) in a disc overexpressing cos2. (E) Same disc as in D, with anti-b-gal in red and overexpressed Cos2 protein
in green. (F) Expression of ptc-lacZ as
shown by nuclear b-gal staining
(red). This swath of nuclei, 8- to 10-cell-diameters wide, is narrower than the
expression of dpp-lacZ shown in A
because ptc-lacZ requires higher
levels of Hh signaling for expression (see text). Hh emanates from en-expressing cells of the posterior compartment
(blue). (G,H) mRNA in situ using an antisense ptc probe in a wild-type (G) and mutant (H) disc in
which costal2–
clones have been generated. Overexpression of cos2 is sufficient to repress ptc transcription in a cell-autonomous manner. (I)
ptc-lacZ expression (red) in a disc
overexpressing cos2. (J) Same
disc as in G, with anti-b-gal in
red and overexpressed Cos2 protein in green.
