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Fig. 2. Wing duplications resulting from S182N expression can be suppressed by co-expression of cos2. (A) Wild-type wing (normal costa indicated by arrow). (B,C) Examples of the two extremes of expressivity seen in wing blades in which S182NGFP is expressed using MS1096 Gal4. The same results are seen in wings expressing S182N. The outgrowth of the costa is shown in B by an arrow. B represents the very mild phenotypes seen, while C represents the extreme phenotypes seen. (D) Overexpression phenotype of MS1096 Gal4; UAS S182T/+ flies. Wing blades are reduced in size and third and fourth wing veins are missing. (E) Phenotype of MS1096 Gal4; UAS cos2GFP/+ flies. There are no signs of anterior wing outgrowth or duplications, but there is the similarity between E and D. (F) Phenotype of MS1096 Gal4; UAS S182N/+; UAS cos2/+ flies. Note there are no signs of anterior wing outgrowth or duplications, and the phenotype is similar to E and D. All flies were grown at 29°C to maximize levels of expression using the MS1906 Gal4 driver, which strongly drives expression throughout the wing pouch. All wings are photographed at the same magnification to show the differences in wing size between each genotype.





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