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Fig. 8. Sequences required for preventing the induction of bap enhancer
activity in the dorsal ectoderm. (A) Summary of tested enhancer derivatives
and their activities in the dorsal mesoderm (ms) and ectoderm (ec). R1, R2 and
R3 denote native motifs with putative repressing activities, whereas tinD1a
and tinD1b denote related sequence motifs from the tinD enhancer of
tinman. (B) Sequence alignments of motifs thought to confer
ectodermal repression from the bap3.2 and tinD enhancers. (C-H)
Dorsal views of early stage 11 ßGal-stained embryos carrying various
reporter constructs (arrow, mesoderm; arrowhead, ectoderm). (C)
bap3.2-lacZ shows almost complete repression in the dorsal ectoderm.
(D) bap3.2.1-lacZ shows complete de-repression in the dorsal
ectoderm. (E) bap3.2
R3-lacZ shows low levels of
de-repression in the dorsal ectoderm (small arrow; see comment in
Fig. 6 legend regarding large
ectodermal cells). (F) bap3.2R1-2-lacZ and (G)
bap3.2R1-3mut-lacZ show strong de-repression of enhancer activities
in the dorsal ectoderm. (H) The addition of tinD1 sequences to
bap3.2.1 prevents ectopic induction in the ectoderm.
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