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Fig. 6. Bmp4 function in lip fusion. (A,B) Whole-mount ß-gal staining (A) and whole-mount in situ hybridization with Bmp2 probe (B) in Bmp4lacZ+/- and wild-type embryos, showing expression of Bmp4 and Bmp2 (arrow) in the fusing region of the lip. (C,D) Whole-mount in situ hybridization with a Bmp4 exon 4 probe showing the absence of a hybridization signal in the Nestin cre;Bmp4 n/f mutant embryo (arrow). (E-H) ß-Gal stained embryos showing cleft lip in the Nestin cre;Bmp4 n/f embryos (arrows). In this experiment the Bmp4lacZ allele was used instead of the Bmp4null allele. Stages and genotypes are labeled.





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