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Fig. 4. Expression and localization of cyclin B1 protein in wild-type (A,C,D,F,G,I) and Ovol1^-/- (B,E,H) testis. Shown are results of immunostaining experiments using ${alpha}$ -cyclin B1 (A-C) and ${alpha}$ -Ldhc4 (D-F) antibodies on P16 (A,B,D,E,G,H) and P21 (C,F,I) testis. (G-I) Merged images. Asterisk in G-I indicates mitotic spermatogonia at the tubule periphery that stained positive for cyclin B1. Arrows and arrowheads indicate cytoplasmic and nuclear cyclin B1 in primary spermatocytes, respectively. Scale bar: 25 µm.

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