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Fig. 7. Protein interactions between MYB23, GL1, TTG1, TRY and GL3 in yeast
two-hybrid assays. lacZ reporter activity in yeast cells for the
pair-wise combination of plasmids is shown. The activity of the negative
control (yeast containing plasmids pACT and pAS) represents the background
level in yeast cells. Interaction between GL3, GL3-96 and TTG1 demonstrates
that the 96 amino acids N-terminal truncated version of GL3 is capable of
interaction with TTG1 and is consistent with previous data
(Payne et al., 2000). Error
bars show standard deviation. AD, GAL4 transcriptional activation domain; BD,
GAL4 DNA binding domain.