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Fig. 1. TRP and Rh1 accumulate in large cytoplasmic vesicles during photoreceptor
maturation. Confocal cross-sections of staged pupal eyes (% pd=% pupal
development) stained for F-actin using rhodamine-phalloidin (red) and either
(A) anti-TRP or (B) anti-Rh1 (green). Actin-rich rhabdomeres stain red at all
stages; overlapping green immunostain (Rh1 or TRP) renders rhabdomeres yellow.
The yellow-red boundary marks the rhabdomere base, the cytoplasmic ends of
microvilli and the origin of the actin-rich rhabdomere terminal web (RTW). (A)
TRP concentrates in large cytoplasmic vesicles during its synthetic peak,
between 50 and 80% pd. (B) Following the onset of Rh1 expression at about 70%
pd, the protein concentrates in large cytoplasmic (Rh1-containing large)
vesicles (RLVs), before it is detected in rhabdomeres at 70% pd. (C) TRP and
Rh1 colocalize in RLVs of 74% pd photoreceptors. (D) A side view of an
ommatidium isolated from a newly eclosed wild-type fly shows RLVs associate
with one or more small dots of F-actin. (E) Most RLVs appear tethered to the
RTW via actin patches. (F) A side view of a live ommatidium isolated from a
6-day-old fly expressing GFP-actin driven by Rh1Gal4. Actin patches decorate
the RTW of living cells. Scale bar: 2 µm in A-D; 1 µm in E; 5 µm in
F.
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