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Fig. 4. Rab11 immunolocalization. (A) In a confocal cross-section of a
newly-eclosed adult eye, triple stained for Rab11 (green), F-actin (red) and
CFP-galactosyl transferase (blue), Rab11 immunolocalizes to vesicles and
tubules distributed throughout the cytoplasm; Rab11-positive vesicles
frequently associate with the RTW (arrows). Rab11 also associates with Golgi
(arrowheads). (B) The cis-Golgi marker, Rab1, localizes to the convex
side of CFP-stained Golgi, while Rab11 localizes to the concave, trans side.
(C) The projection of five 0.3 µm interval serial z-sections of a
30% pd photoreceptor Golgi triple stained for Rab1, Rab11 and the CFP-Golgi
marker localizes Rab11 to trans-Golgi. Arrows show the edges of staining. (D)
The time course of Rh1 and Rab11 localizations after blue light irradiation.
Arrows and arrowheads indicate colocalization of Rab11 and Rh1 at the
rhabdomere base and in cytoplasmic organelles. (E) Rh1 and Rab11 intensely
colocalize at the base of rhabdomere 60 minutes after blue light. (F) Most of
Rh1 positive organelles are Golgi bodies. Scale bar: 2 µm in A-E.
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