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Fig. 5. Rh1 transport requires Rab11. (A) Rab11-reduced photoreceptors (arrows) in a mosaic eye triple stained for Rh1 (blue), Rab11 (green) and F-actin (red) show disrupted RLVs and Rh1 dispersed throughout the photoreceptor cytoplasm. Rh1 accumulation in rhabdomeres and rhabdomere size is decreased in the Rab11-reduced photoreceptors. (B) Electron micrographs of mosaic ommatidia show photoreceptors with normal MVBs (arrows) and vesicle-filled cells lacking MVBs (arrowheads). Other internal membranes appear normal. (C,D) Higher magnification of abnormal cytoplasmic vesicles shows unusual irregular MVB vesiculation. These appear incomplete, leaving openings, or iterated, resulting in vesicles within endovesicles not seen in normal MVBs. Scale bar: 5 µm in A; 2 µm in B; 500 nm in C,D.





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