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Fig. 6. Rab11 RNAi inhibits Rh1 transport. (A) In control Hs-Gal4 flies, heat
shocked at 27% pd, then fixed and stained at 85% pd, Rh1 (blue) transport is
normal. Rh1 is present in cytoplasmic RLVs and in rhabdomeres stained red
using rhodamine phalloidin. Rab11-positive (green) vesicles are distributed
throughout the cytoplasm. (B) In experimental Hs-Gal4, dsRNA flies, Rh1 is
dispersed throughout photoreceptor cytoplasm and delivery to the rhabdomere is
inhibited. (C) Shown separately, Rab11 immunostaining of control flies is
brighter than D, Hs-Gal4, Rab11 dsRNA flies. The R2 photoreceptor (arrow)
shows a higher level of Rab11 signal and detectable Rh1 delivery to the
rhabdomere. R7, which also shows Rab11 signal here, does not express Rh1;
transport of its rhodopsin, either Rh3 or Rh5, cannot be determined. (E)
Electron micrographs of Hs-Gal4, UAS-Rab11 dsRNA pupae. Rhabdomere
size is small. Abnormal cytoplasmic vesicles (arrowheads) proliferate and MVBs
are absent. Scale bar: 2 µm in A-E.
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