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Fig. 1. Targeted disruption of the nectin 1 and nectin 3 genes. (A) Targeted
disruption of the nectin 1 gene. (A) (a) The structure of the mouse nectin 1
gene with coding exons 2-5 is shown at the top. A targeting vector was
designed to remove the exon 2. The construct contained 2.8 kb 5'
flanking sequence and 4.0 kb 3' flanking sequence. (b) Southern blotting
of ES clones. HindIII-digested DNAs derived from ES cells were
hybridized with the 5'- or 3'-probe. WT, wild-type; +/–,
nectin 1+/–. (c) Genotyping by PCR of genomic DNA extracted
from littermate mice at 21 days of age. Wild-type (WT) and targeted alleles
give the bands of 639 bp and 459 bp, respectively. +/–, nectin
1+/–; –/–, nectin 1–/–.
(d) Western blotting of the extracts from the brains of wild-type and nectin
1–/– mice with the anti-nectin 1 and anti-actin
antibodies. (B) Targeted disruption of the nectin 3 gene. (a) The structure of
the mouse nectin 3 gene with coding exon 1 is shown at the top. A targeting
vector was designed to remove the exon 1. The construct contained 6.5 kb
5' flanking sequence and 4.2 kb 3' flanking sequence. (b) Southern
blotting of ES clones. BamHI-digested DNAs derived from ES cells were
hybridized with the 5'- or 3'-probe. WT, wild-type; +/–,
nectin 3+/–. (c) Genotyping by PCR of genomic DNA extracted
from littermate mice at 21 days of age. Wild-type and targeted alleles give
the bands of 379 bp and 814 bp, respectively. WT, wild-type; +/–, nectin
3+/–; –/–, nectin 3–/–.
(d) Western blotting of the extracts from the brains of wild-type and nectin
3–/– mice with the anti-nectin 3 and anti-actin
antibodies.
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