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Fig. 2. Isolation of the ROXY1 gene. (A) T-DNA insertion sites are shown
for each allele. The T-DNA insert in roxy1-1 is located in the
promoter, 175 bp upstream of the start codon ATG. roxy1-2 and
roxy1-3 carry insertions in the single coding exon, 91 and 291 bp
downstream of the ATG, respectively. Numbering of coordinates is based on the
Arabidopsis genomic clone BACF1C9 that contains the Col-0
ROXY1 gene. Left and right (L and R) T-DNA borders are only indicated
where they could be identified. (B) ROXY1 cDNA and predicted amino
acid sequence encoding a 136 amino acid protein (GenBank Accession Number
AY910752). The dithiol CCMC and the monothiol CSGS motif are underlined. Locus
name is At3g02000. (C) Comparison of ROXY1 expression in wild-type
and roxy1-2 and roxy1-3 mutants by RT-PCR. RNA was isolated
from inflorescences and primers were used allowing specific cDNA
amplification. As a control, reactions with 18S rRNA were carried out.
