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Fig. 6. Changes in AG expression in roxy1-3 ap1-10 double mutants. (A) ap1-10 mutants develop leaf-like first whorl organs; petals are absent in the second whorl, but an additional flower is formed in flowers arising on the inflorescence at later stages. (B) Strong feminization of the first whorl organs in the roxy1-3 ap1-10 double mutant (arrow). (C) In situ analysis of AG expression in young ap1-10 mutant inflorescences. Onset of AG expression is detectable at stage 3 and is confined to the two inner floral whorls. (D) AG is prematurely expressed in the roxy1-3 ap1-10 double mutant and expression is already detectable in the inflorescence meristem (im). Furthermore, AG is ectopically expressed in sepal primordia at stage 3. (E,G) AG-I::GUS expression in ap1-10 flowers resembles wild-type AG expression, being confined to the third and fourth whorl organs. (F,H) AG-I::GUS analysis in inflorescences and mature flowers of roxy1-3 ap1-10 mutants shows continued ectopic AG expression in the feminized first whorl organs (arrow).





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