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Fig. 2. Localization of a wing-specific regulatory element of knot. (A)
Genomic region upstream of the knot gene. The first exon of
knot is shown in blue. Exons of neighboring genes are shown in gray.
Regions 10-15 kb upstream of the transcription start site were tested for
cis-regulatory activity. Regions directing expression in the wing are
indicated in blue and regions lacking activity are indicated in black. (B)
VISTA plot (Couronne et al.,
2003) of knMel1-2330 fragment aligned with
sequence from D. pseudoobscura and D. virilis. A window size
of 50 bp was used, and regions that are greater than 70% identical are
indicated in orange. Shown below are the constructs generated on the basis of
the distribution of sequence conservation among Drosophila. (C)
lacZ reporter expression in the wing imaginal disc under the control
of the knMel701-1991 fragment. No expression is observed
in the haltere imaginal disc (inset). (D) Wing imaginal disc of individual
carrying knMel701-1991 fragment stained for Knot protein
(red) and lacZ (green). The yellow stripe indicates complete overlap
of the two patterns.
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