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Fig. 5. UBX-binding sites and an additional site contribute to repression of knot in the haltere. (A) Mutation of UBX site 1 alone in kn^Mel701-1991 (kn^Mel701-1991UBX1KO) does not cause any de-repression of reporter gene expression in the haltere. (B) Mutation of 10 UBX-binding sites in kn^Mel701-1991 (kn^Mel701-1991KO) causes partial de-repression of reporter gene expression in the haltere. (C) kn^Mel701-1878, which contains 43 nucleotides not present in kn^Mel701-1835 but no additional UBX-binding sites, directs weak reporter gene expression in the haltere, indicating that repression is partially independent of UBX. (D) Point mutation of kn^Mel1834-1837 (kn^Mel701-1991mut) results in partial de-repression. (E) Mutation of both kn^Mel1834-1837 and the UBX-binding sites (kn^Mel701-1991KOmut) results in full de-repression in the haltere.

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