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Fig. 6. Functional UBX binding sites are not conserved within Drosophila (A) Schematic of a 1.9 kb fragment of D. pseudoobscura DNA, indicating the position of putative Ci-binding sites in blue and UBX TAAT core sequences in brown. This fragment (kn^Pse1-1935) and a derivative that truncates 300 nucleotides from the 3' end (kn^Pse1-1643) were cloned from D. pseudoobscura and injected into D. melanogaster. (B) Alignment of knot enhancer sequences from five Drosophila species: D. melanogaster, D. mauritiana, D. biarmipes, D. malerkotliana and D. pseudoobscura. (Above) Aligned block 1, which contains the footprinted UBX binding sites (red), the functional Ci binding site (blue) and the mutated site at the boundary of the kn^Mel701-1835 construct (purple). (Below) Aligned block 2, with conserved TAAT sequences indicated in red. Arrows in aligned block 2 indicate the kn^Mel2499-2722 sequence added to kn^Mel701-1835 to generate the kn^Melcomposite construct.

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