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Fig. 7. D. melanogaster possesses a second UBX-responsive knot regulatory element that is conserved with D. pseudoobscura. (A) kn^Pse1-1935 is expressed in the wing, but not in the haltere. (B) kn^Pse1-1643, which eliminates the conserved block of TAAT core sequences, is expressed in both the wing and the haltere. (C) Addition of 222 nucleotides (kn^Mel2499-2722) of D. melanogaster sequence to the de-repressed kn^Mel701-1835 construct (to generate kn^Melcomposite) is sufficient to restore repression, indicating that this conserved region is a functional repressor sequence in both D. melanogaster and D. pseudoobscura. (D) Mutation of UBX sites alone in kn^Melcomposite (kn^MelcompositeKO) is sufficient for full de-repression, suggesting that UBX does not require the function of a collaborator at this repressor element. (E) The knot wing regulatory region. The crucial Ci binding site is indicated in dark blue, the UBX repressor element novel to the D. melanogaster lineage is indicated in light blue, the conserved UBX repressor element is indicated in green and the collaborating repressor site is indicated in pink.

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