|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 2. pitx3 and dlx3b form overlapping expression domains at
the anterior neural plate border. (A-D) Embryos labeled with probes for
pitx3 (arrowheads) and (A) zic1, (B) foxb1.2; (C)
pax6a or (D) hgg1 (red). (E) Anterior neural plate border
cells express dlx3b. (E, inset) Single cell injected with
fluorescein-dextran, labeled for lineage tracer (red). (F-H) A subset of cells
co-expresses: (F) pitx3 (blue) and dlx3b (red); (G)
bmp2b (blue, non-neural ectoderm), shh (blue, neural plate
midline) and hgg1 (red, polster); (H) dlx3b (red) and
eya1 (black). (I) Sketch of anterior neural plate gene expression
domains (pitx3, blue, n=12; dlx3b, red,
n=15; zic1, foxb1.2, otx2, grey)
(Varga et al., 1999) to
predict location of pitx3-expressing cells in live unlabeled embryos.
Averaged gene expression domains; bars indicate standard deviations from the
mean, relative to neural plate midline and center of polster (asterisk). All
panels at bud stage, except C, which is at the one-somite stage. Dorsal views
of prospective head region, ventral towards the top. Scale bars: 100
µm.
|
