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Fig. 6. Pitx3 is required for pitx3 expression at bud stage, and Dlx3b and
Dlx4b restrict pituitary pre-placode size during mid-somitogenesis. (A-I)
Control and morpholino injected embryos, labeled with pitx3 probe at
bud stage (tb, A-C), 18-somite stage (D-F) and prim-5 stage (24 h; G-I).
(B,E,H) pitx3 morpholino injection leads to reduced pitx3
expression in placodal field (B; n=23/30), in pituitary pre-placode
(E; n=33/46) and in pituitary placode (H; n=43/68). (C,F,I)
Injection of dlx3b and dlx4b morpholinos leads to reduction
of pitx3 expression in prospective lens precursors (arrowheads in C;
n=29/38), and an expansion of the pituitary pre-placode (F, white
bar; n=23/30) and pituitary placode size (I; n=32/40;
arrowheads: ectopic pitx3 expression). (A-C) Dorsal view, ventral
towards the top. (D-I) Frontal view, dorsal towards the top. Scale bar: 100
µm.
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