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Fig. 6. Midgut expression comparisons between several enhancers regulated by Lab.
(A) Sequences of the Lab/Exd-binding sites of enhancers thought to be
regulated by Lab (red) and its co-factors Exd (blue) and Hth (bold). The two
central nucleotides of the Lab/Exd-binding site are highlighted in yellow.
These enhancers are derived from the mouse Hoxb1 (repeat3),
or the Drosophila Lab (lab550 and lab48/95) genes.
The sequence of the divergent Lab/Exd/Hth-binding site of the EVIII
enhancer of CG11339 is also shown for comparison. (B-I) All embryos
are shown at stages 12 and 14. Confocal analysis of Lab (immunostaining;
green) and ß-gal (immunostaining; red) expressions in lines carrying the
lacZ transgene under the control of enhancers lab550 (B,C),
lab48/95 (D,E), repeat3 (F,G) and EVIII of
CG11339 (H,I).
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