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Fig. 5. Gene regulation in Alx4, Cart1, Gli3 and Tbx15 compound
mutants. (A-D) Pax1 expression in E10.5 wild-type (A),
Tbx15–/– (B),
Gli3–/– (C) and Tbx15/Gli3
double mutant (D) forelimb buds. Note the downregulation of Pax1
expression in the mutants. (E-L) Pax1 expression in E10.75 wild-type
(E,I), Alx4–/– (F),
Cart1–/– (G),
Alx4+/–/Cart1–/– (J)
and
Alx4–/–/Cart1–/–
(H,K,L) forelimb buds. (E-H) Dorsal views of the limb buds. Note the
downregulation of Pax1 in Alx4/Cart1 compound
mutants (H). (I-L) Ventral view of the limb buds. Yellow arrowhead indicates
the shift of Pax1 expression in
Alx4+/–/Cart1–/– (J)
and
Alx4–/–/Cart1–/–
(K,L) mutants. (M-P) Pax1 expression in wild-type (M,O) and
Alx4/Cart1 mutant (N,P) fore- and hindlimb (HL) buds at
E11.5. Yellow arrowhead (N,P) indicates the shift of Pax1 expression.
(Q-T) In situ hybridization on serial transversal sections at E10.5 of
wild-type (Q,S) and Alx4/Cart1 mutant (R,T) embryos. (Q,R)
Pax1 expression is downregulated in Alx4/Cart1
mutants. (S,T) Pax3 is abnormally expressed in the mesenchyme just
adjacent to the surface ectoderm in Alx4/Cart1 mutants
(black arrow in T). (U,V) Whole-mount in situ hybridization with Pax3
probe of E10.5 wild-type (U) and Alx4/Cart1 mutant (V) limb
buds. Red arrowhead (V) indicates Pax3 expression that is shifted
towards the antero-proximal region of the limb bud. (W,X) Expression of
Scleraxis (Scx) in E10.5 wild-type (W) and
Alx4/Cart1 mutant (X) limb buds. Green arrowhead (X)
indiactes the shift of Scx expression in the
Alx4/Cart1 mutant. (Y) Schematic representation of the
expression patterns of Pax1, Pax3 and Scleraxis in wild
type, and in Alx4/Cart1 and Tbx15/Gli3
compound mutants.
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