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Fig. 3. Altered distribution of RA-responsive cells in Raldh2^L–/– brachial spinal cord. (A-F) Immunodetection of ß-Gal (red) on transverse ventral spinal cord sections (A-D) and dissected spinal cords (E-F) in RARE_hsp68_lacZ transgenic mice. (C,D) Combined immunodetection of ß-Gal (red) and Islet1/2 (green). Arrows point to ß-Gal+/Islet1/2+ cells (orange). Brackets in (F) indicate abnormal ß-Gal distribution in a broad longitudinal band within the intermediate region. (G,H) Rarb transcript distribution (red) combined to Raldh2 immunofluorescence (green) on flat-mounted spinal cords. (G) Composite image in which the overlay of the two signals is shown on the left side only. The dotted lines in (G,H) point to the thoracic MMC. (I,L) Rarb transcripts on transverse ventral spinal cord sections at two different AP levels as indicated in E,F. Arrowheads point to Rarb+ motor columns and asterisks show a deficiency of Rarb+ cells in mutants compared with controls. i, intermediate region; vh, ventral horns.

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