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Fig. 2. kis encodes multiple large nuclear proteins that are subunits of
distinct complexes. (A) Embryo and salivary gland extracts were resolved on a
5% SDS-polyacrylamide gel and analyzed by western blotting. Molecular masses
were determined relative to prestained markers and cytoplasmic dynein
(detected by western blotting). Antibodies against the common C-terminal
segment detect both KIS-L and KIS-S in embryo extracts (lane 1) but only KIS-L
in salivary gland extracts (lane 2). Antibodies against the N-terminal segment
unique to KIS-L recognize only this protein in embryo extracts (lane 3). The
asterisk marks minor bands that are occasionally observed and probably
represent degradation products or minor isoforms of KIS. (B)
Drosophila embryos were stained with antibodies that specifically
recognize KIS-L. DNA was visualized using propidium iodide. There is uniform
nuclear distribution of KIS-L. (C) Native embryo extracts were fractionated on
a Superose 6 gel filtration column and assayed for chromatin-remodeling
factors by western blotting. Fraction numbers are indicated at the top. Void
and elution volumes of native molecular mass standards are shown by vertical
arrows. Denatured molecular masses of the proteins are indicated on the
right.
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