DEV01715 Supplementary Material

Files in this Data Supplement:

• Supplemental Table 1 (Adobe PDF)
• Supplemental Table 2 (Adobe PDF)
• Supplemental Table 3 (Adobe PDF)
• Supplemental Table 4 (Adobe PDF)
• Supplemental Figure 1 -

  Fig. S1. Histone modifications in female XEN and TS cells. Representative images show immunofluorescent detection of histone modifications H3 di-methyl K4 and H3 di-methyl K9, combined with Xist RNA FISH. Immunodetections are in red and Xist RNA in green. XEN cells (A) and TS cells (B) show depletion for H3 di-meK4, in agreement with the H3 acK9 depletion (Fig. 6). H3 di-meK9 is neither enriched nor depleted on the inactive X chromosome in XEN or TS cells.

• Movie 1 -

  Movie 1. Videomicroscopy of XEN cells. GFP-IM8A1-1 cells were cultured on gelatin in the presence of EMFI-CM. Phase-contrast micrographs were taken every 2 minutes for 12 hours; 100 minutes of this footage is shown here. Cells-of-interest, labeled in blue and red, did not undergo cell division during the 100-minute period. The blue cell migrates between two cells by sending out a typical lamellipodial process, but then continues to migrate as a rounded cell. It then sends out a long pseudopodium and retracts it. The red cell starts out epithelioid, but quickly rounds up and migrates in this configuration. It also produces a pseudopodium and retracts it before it reverts to an epithelioid state.