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Fig. 1. The extra-embryonic endoderm lineage and XEN cell cultures. (A) Schematic
of an E4.5 blastocyst and an E5.5 embryo, illustrating the primitive, visceral
and parietal endoderm (green). The trophoblast and epiblast lineages are
represented in yellow and red, respectively. The proximodistal axis is
indicated. ICM, inner cell mass. (B) Phase-contrast micrograph of XEN cells
cultured at low density illustrating the refractile, rounded cell type and the
epithelioid cell type. (C) XEN cells grown to near confluency showing the
presence of individual cells and an epithelial sheet of cells. (D) XEN cells
participating in a lattice-type structure. (E) XEN cells plated in the absence
of gelatin resulted in differentiation of some cells into large vacuolated
cells in 6 days. Scale bar: 10 µm for B-D; 40 µm for E.
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