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Fig. 3. RT-PCR expression analysis of XEN cells. cDNA was prepared from ES cells,
embryoid bodies (EB) at day 5 (D5) and day 7 (D7), and three different XEN
cell cultures. XEN cells were either cultured on gelatin in maintenance
conditions (XEN) or differentiated (Diff) by removal of EMFI-CM and gelatin
for 4 or 8 days (D4, D8). Hnf4 and Foxa2 were consistently
detected in all XEN cell cultures, while Gata4 and Sox7
decreased expression upon differentiation. By contrast,
-fetoprotein
(Afp) increased with differentiation. Oct4 was undetectable
in XEN cells.