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Fig. 5. X-inactivation in ES, TS and XEN cells. Quantification of Xist
transcripts by real-time RT-PCR in (A) ES cells and (B) XEN and TS cells
normalized using the endogenous gene 18S. (C) Quantification of
Nap1l2 transcripts normalized using the Rrm2 gene in XEN
cells. In differentiated ES cells (A), random X-inactivation leads to
equivalent levels of Xist expression from the 129 and Pgk alleles. By
contrast, in female XEN cells (GHP7/3 and GHP7/9), Xist is expressed
only from the paternal Pgk allele (B) and Nap1l2 only from the
maternal 129 allele (C), indicating that the paternal X chromosome is
preferentially inactivated in female XEN cells, just as in female TS cells
(where Xist is expressed only from the paternal 129 allele; B). In
control male XEN cells (GHP7/7), the single X chromosome derived from 129 is
active, as shown by the absence of Xist (B) and presence of
Nap1l2 expression (C).
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