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Fig. 4. Introduction of mutations into the BSs for Lhx, Fox and T-box proteins
resulted in a loss of lacZ transcription in the vegetal hemisphere.
(A-D) Frequency of lacZ-positive cells for a given cell in 64-cell
embryos injected with reporter constructs harboring the regulatory modules
indicated on the left side. Red, yellow and blue ovals represent the binding
sites for Lhx, Fox and T-box proteins, respectively. (E) Frequency of
lacZ-transcribing cells for a given cell lineage in 64-cell embryos
injected with reporter constructs harboring the DNA fragments shown on the
left side. DNA fragment Lhx/Fox-BS contains the single Fox-binding site and
the two Lhx-binding sites from regulatory module #6. DNA fragments Lhx-BS and
Fox-BS include four binding sites for Lhx and Fox, respectively, which are
from regulatory module #6. The DNA fragment T-box-BS includes four copies of
the T-box-binding site present in the regulatory module #3. The nucleotides
marked yellow, red and blue represent the putative binding sites for Fox, Lhx
and T-box proteins, respectively. For color codes in histograms, see
Fig. 1.
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