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Fig. 5. The regulatory modules of Hr-Otx are capable of directing
line-specific transcription in Ciona intestinalis embryos. The
reporter constructs containing regulatory modules #2 (A), #3 (B), #4 (C,D), #5
(E,E') and #6 (F) were electroporated into Ciona fertilized
eggs, and the resulting embryos were examined for lacZ transcripts at
the 64-cell (A-C,E,E',F) or 76-cell (D) stage. Animal (A,E) and vegetal
(B-D,E',F) views are shown. Shown below the images are the identity of
the lacZ-expressing cell line in the Ciona embryo and a
schematic representation of the in situ hybridization specimen. (G,H)
Frequency of lacZ-positive cells for a given cell line in 64-cell (G)
or 76-cell (H) embryos injected with reporter constructs harboring the
regulatory modules indicated on the left side. For color codes, see
Fig. 1.
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