DEV01720 Supplementary Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1. Early development of a wild-type Drosophila embryo. Anterior is towards the left and dorsal is upwards. At the beginning of the movie, the cortex retracts from the vitelline membrane at the posterior pole, giving rise to a clear space. This space is filled later by the budding pole cells. The cytoplasmic movements and the rhythmic contractions of the embryo are associated with every nuclear cleavage cycle in the syncytial blastoderm. Contractions start simultaneously at the anterior and posterior pole. When cellularization starts, a sharp line that represents the actin network at the tip of the ingrowing plasma membrane starts to move from the surface membrane into the interior of the embryo. At the end of cellularization, gastrulation movements begin.

• Movie 2 -

  Movie 2. Early development of a PTEN^DJ189 mutant embryo derived from a germ-line clone. Anterior is towards the right and dorsal upwards. In contrast to wild type, the posterior cortex does not retract from the vitelline membrane and pole cells do not form. Mitotic waves are always initiated at the posterior pole and sometimes stop in the middle of the embryo. The cellularization front moves much faster in the anterior region of the embryo than in the posterior region. Cellularization at the posterior pole is not yet finished when gastrulation movements start.