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Fig. 1. PDZ domains 2 and 3 of Baz bind directly to the C-terminal PDZ-binding
motif of PTEN2 in the yeast 2-hybrid system. (A) In the yeast 2-hybrid screen
using the three PDZ domains of Baz as bait, we isolated three independent
clones corresponding to the C terminus of PTEN2. One clone contains amino
acids 316-511 and two clones contain amino acids 297-511 of PTEN2. A deletion
mutant lacking the three C-terminal amino acid residues of PTEN2 did not bind
to the PDZ domains of Baz, nor did PTEN isoform 3, which differs only at the C
terminus from PTEN2 and lacks the PDZ-binding motif. (B) Different regions of
Baz were tested for interaction with the C terminus of PTEN2 and with the
C-terminal deletion mutant of PTEN2 lacking the PDZ-binding motif. The
smallest fragment that interacted with PTEN2, but not with the mutant lacking
the PDZ-binding motif, contained PDZ domains 2 and 3.
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