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Fig. 3. Colocalization of PTEN2 and Baz in embryonic epithelia and neuroblasts is
dependent on the PDZ-binding motif of PTEN2. (A) An embryo expressing PTEN2
ubiquitously under control of a maternal GAL4 driver was stained with
antibodies against PTEN and Baz. Both proteins colocalize in the apical cortex
of the epidermis (arrows) and in the apical cortex of neuroblasts (asterisks).
(B) At later developmental stages, apical colocalization of PTEN2 and Baz in
epithelia is even more pronounced. Neurotactin (blue, right panel) was used as
a marker for the basolateral membrane. No overlap between PTEN and Neurotactin
is visible. (C) The PTEN3 isoform, which lacks the PDZ-binding motif at the C
terminus but is otherwise identical to PTEN2 is localized on the whole plasma
membrane and in the cytoplasm. PTEN3 staining clearly overlaps with
Neurotactin on the basolateral membrane (purple, right panel). Apical
localization of Baz is unaffected by overexpression of PTEN3. (D)
PtdIns(4,5)P2 colocalizes with Baz in the embryonic
epidermis. We expressed a PLC
-PH-GFP fusion protein under control of a
ubiquitous maternal promoter and stained these embryos with an antibody
against Baz. The PLC-PH-GFP fusion protein is present on the whole
plasma membrane except for the free apical surface and is strongly enriched in
the most apical region of the lateral plasma membrane where it colocalizes
with Baz. The embryos shown are at stage 10 (A) and at stage 13 (B-D)
(Campos-Ortega and Hartenstein,
1997). Genotypes are: P{w
+mC=matalpha4-GAL-VP16}V67;UAS
Pten2 (A); P{w
+mC=Act5C-GAL4}17bF01;UAS Pten2 (B);
P{w +mC=Act5C-GAL4}17bF01/UAS
Pten3 (C); and P{w
+mC=matalpha4-GAL-VP16}V67/UAS
PLC-PH-GFP (D). Scale bar: 20 µm.
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