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Fig. 5. Pten mutant embryos show defects in nuclear migration, cell cycle
regulation and in organization of the actin cytoskeleton during
cellularization. (A,A',C,C') Cortical nuclei in wild-type embryos
are evenly spaced and divide in a nearly synchronous pattern. (B,B') In
Pten mutant embryos, nuclear density in the posterior region of the
embryo is much lower than in the anterior region because of a defect in
nuclear migration in pre-blastoderm stages. (D,D') At syncytial
blastoderm, nuclei are still unevenly spaced and divide asynchronously. (A-D)
Whole embryos stained for DNA; (A'-D') higher-magnification images
of the same embryos as shown in A-D, stained for DNA (green) and
-tubulin (blue). The arrowheads in B,B',D,D' are in
corresponding positions to illustrate which region of the embryo is shown at
higher magnification. (E,E',G,G') During cellularization of
wild-type embryos, a regular network of actin filaments (red) forms at the
front of the ingrowing plasma membrane. (F,F',H,H') In
Pten mutant embryos, the cellularization front is very uneven.
Cellularization is particularly slow at the posterior pole where the pole
cells have failed to form (F',H'). Arrows in F,F',H,H'
indicate borders between nuclei at different cell cycle stages. In all images,
anterior is towards the left. Scale bar: 100 µm in A-H; 20 µm in
A'-H'.
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