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Fig. 6. Subcellular localization of VAN3. (A) Arabidopsis
suspension-cultured cells. (B-D,G-I) Location of VAN3–Venus (red) and
GFP-tagged subcellular marker genes (green). 35S-promoter-driven Venus-tagged
VAN3 and GFP-tagged subcellular markers were co-introduced into protoplasts of
Arabidopsis suspension-cultured cells. (B-D) Merged image of
VAN3–Venus and the ER marker HDEL–GFP (B), the cis-Golgi marker
SYP31–GFP (C), the endosome marker ARA6–GFP (D). (E) Location of
ARA7–GFP in the gnom mutant cells. (F) VAN3–Venus in
gnom mutant cells. (G–I) Localization of the TGN marker
SYP41–GFP (G) and VAN3–Venus (H), and a merged image of G and H
(I). E and F are projection images of serial confocal planes, A-D and G-I and
single confocal slice images. Scale bars: (A-I) 5 µm.
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