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Fig. 4. Super-repressor I{kappa}B-{alpha} reduces neurite growth from nodose neurons in a developmentally dependent manner. E16, E18, P0, P1 and P3 nodose neurons were transfected with a YFP expression plasmid together with either a super-repressor I{kappa}B-{alpha} plasmid or an empty control plasmid and were incubated in medium containing 10 ng/ml BDNF. (A) Percent survival 48 hours after transfection. In addition to showing survival data for super-repressor I{kappa}B-{alpha} transfected and control transfected neurons grown with BDNF, survival data for control transfected neurons grown without BDNF are also shown at each age. (B) Total neurite length 24 hours after transfection. (C) Number of branching points in neurite arbors 24 hours after transfection. (D-H) Sholl analysis of neurite arbor morphology 24 hours after transfection at E16, E18, P0, P1 and P3, respectively. Statistical comparisons shown are with respect to the control transfected neurons, *P<0.05, **P<0.001.





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