DEV01709 Supplementary Material

Files in this Data Supplement:

• Supplemental Table (Adobe PDF)
• Supplemental Figure 1 -

  Fig. S1. Genomic structures of the PpLFY1 and PpLFY2 loci in PpLFY1-GUS and PpLFY2-GUS lines and the confirmation of targeting by Southern analyses. The PpLFY1 (A) and PpLFY2 (B) loci in PpLFY1-GUS and PpLFY2-GUS lines, respectively. The boxes indicate exons and the lines between the boxes introns; circles, putative start codons and squares, putative stop codons. b, BglII; x, XhoI; uidA, uidA coding region; nos-ter, nopaline synthase polyadenylation signal; NPTII, NPTII expression cassette. The sense directions for uidA and nptII are indicated with arrows. The scale bar is the same in A and B. (C,D) Southern analyses for PpLFY1-GUS (C) and PpLFY2-GUS (D) lines. Genomic DNA of the wild type (w) and of the PpLFY1-GUS-1 to 3 and PpLFY2-GUS-1 to 8 lines were digested with XhoI (C) or BglII (D). The probe used is indicated below each image.

• Supplemental Figure 2 -

  Fig. S2. Genomic structures of the PpLFY1 and PpLFY2 loci in the PpLFY disruptants and the confirmation of targeting by Southern analyses. The PpLFY1 (A) and PpLFY2 (B) loci in PpLFY1-dis, PpLFY2-dis and PpLFY1-PpLFY2-dis lines. The boxes indicate exons and the lines between the boxes indicate introns; circles, putative start codons and squares, putative stop codons of PpLFY genes. e, EcoRI; v, EcoRV; HPT, HPT expression cassette; NPTII, NPTII expression cassette. The sense directions of hpt and nptII are indicated with arrows. The scale bar is the same in A and B. (C-E) Southern analyses for the PpLFY1 (C,E) or PpLFY2 (D) loci in PpLFY1-dis (C), PpLFY2-dis (D) and PpLFY1-PpLFY2-dis (D,E) lines. Genomic DNA from the wild type (w), PpLFY1-dis-1 to 8, PpLFY2-dis-1 and PpLFY1-PpLFY2-dis-1 to 6 lines were digested with EcoRV or EcoRI and hybridized with the probes indicated below each image.