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Fig. 5. Raldh3 overexpression in the presence of ATR reproduces the effects of RA
treatments on eye DV polarity. (A) Embryos were bilaterally injected with a
total of 1 ng Raldh3 mRNA and treated with 0.5 µM ATR from stage
12.5/13, followed by molecular marker analysis at stage 33. The combination of
Raldh3 and ATR expands Pax2 expression, while ATR alone has only a
weak effect. A comparable expansion of Pax2 is obtained by a dose of
2 µM ATR in the absence of exogenous Raldh3. (B) The combination of 4 ng
Raldh3 mRNA and 2.5 µM ATR can upregulate Vax2 in the DR,
while ATR alone has only a weak effect. Strong Vax2 upregulation is
also caused by a dose of 10 µM ATR in the absence of exogenous Raldh3, as
shown in both lateral (upper row) and anterior view (lower row).
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