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Fig. 6. RA, Hh and FGFR signaling pathways can partially ventralize the eye field at neurula stages. (A) In embryos treated with 10 µM RA from stage 12.5/13 (right column), the lateral region of Vax2 domain (indicated by the yellow brackets) is expanded at late neurula stages with respect to control embryos, while Vent2 domain is reduced. (B) Mid-late neurula embryos unilaterally injected either with 500 pg bhh mRNA or with 2.5 pg iFGFR1 mRNA, followed by induction with AP20187 from stage 12.5/13. Compared with the uninjected side, Vax2 expression is expanded, while ET is repressed. The broken yellow lines indicate the embryo midline.





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