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Fig. 8. FGFR and Hh signaling can collaborate in eye ventralization. (A) Embryos
were unilaterally co-injected with 1 pg bhh mRNA and 0.5 pg
iFGFR1 mRNA, and induced with AP20187 from stage 12.5/13, followed by
molecular marker analysis at stage 33. Pax2 is mainly activated
within the ventral half of the eye (class I eyes) by bhh alone, while
in the presence of iFGFR1 and bhh Pax2 expression extends
into the dorsal eye (class II and class III eyes). Co-injection of
bhh and iFGFR1 mRNAs also slightly increases the frequency
of eyes with ubiquitous Vax2 expression and more strongly represses
Tbx5 when compared with bhh alone. Under these conditions,
iFGFR1 mRNA alone has no obvious effect on Pax2, Vax2 and
Tbx5 expression. (B) Quantification of the effects of bhh
and iFGFR1 on Pax2, Vax2 and Tbx5 expression.
Between 24 and 30 embryos were analyzed for each sample in these experiments.
(C) Schematic representation of the results shown in A. In class II eyes, the
expanded OS does not reach the most dorsal retina, which can acquire in part
ventral identity. In class III eyes, the retina is mostly or completely
transformed into OS.
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