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Fig. 2. wnt3a and wnt8 are required for the maintenance, not initiation, of tailbud marker expression. (A-L) Embryos injected with the indicated morpholinos were fixed at tailbud stage and stained for ntl (A-D), fgf8 (E-H) and tbx6 (I-L). Embryos are shown in dorsal views, anterior to the top. (M-B') Injected embryos were allowed to develop until the 5-somite stage (M-P) or 10-somite stage (Q-B') and stained for tbx6 (M-T), spt (U-X) or ppt (Y-B'). All embryos are flat mounted and shown in dorsal views with anterior to the top. By the 10-somite stage in the tailbud of wnt3a/wnt8 MO embryos, tbx6 (T) and spt (X) are not expressed, while ppt (B') is still expressed at reduced levels compared with wnt3a MO (Z) or wnt8 MO (A').





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