DEV01782 Supplementary Material

Files in this Data Supplement:

• Supplemental Data Files - Self Extracting Archive, with readme file
• Supplemental Figure 1 -

  Fig. S1. QT cluster analysis of microarray data for three genotypes across 10 time points (Baugh et al., 2003; Heyer et al., 1999). Two-hundred clusters are shown, with line width proportional to the log of the number of genes in each cluster. y-axis indicates normalized expression levels and the x-axis indicates time relative to the four-cell stage. Wild type (N) is black, mex-3(zu155); skn-1(RNAi) (ms) is red and pie-1(zu154) (pm) is blue.

• Supplemental Figure 2 -

  Fig. S2. Wild-type and mutant temporal expression patterns for validated target genes. Expression levels in wild type, pie-1(zu154), and mex-3(zu155); skn-1(RNAi) are shown for 22 validated target genes (A-V). Three-letter gene names are used for characterized genes, while ORF names are used for uncharacterized genes. Transcript abundance (arbitrary units) is plotted on the y-axis and minutes after the four-cell stage on the x-axis.

• Movie 1 -

  Movie 1. Three-dimensional projection of pal-1::YFP reporter expression 210 minutes after the four-cell stage (22°C; ~200 cells).

• Movie 2 -

  Movie 2. Three-dimensional projection of vab-7::GFP reporter expression 210 minutes after the four-cell stage (22°C; ~200 cells).

• Movie 3 -

  Movie 3. Three-dimensional projection of cwn-1::YFP reporter expression 210 minutes after the four-cell stage (22°C; ~200 cells).

• Movie 4 -

  Movie 4. Three-dimensional projection of C55C2.1::YFP reporter expression 210 minutes after the four-cell stage (22°C; ~200 cells).

• Movie 5 -

  Movie 5. Three-dimensional projection of unc-120::YFP reporter expression 210 minutes after the four-cell stage (22°C; ~200 cells).

• Movie 6 -

  Movie 6. Three-dimensional projection of hnd-1::GFP::LacZ reporter expression 210 minutes after the four-cell stage (22°C; ~200 cells).

• Movie 7 -

  Movie 7. Three-dimensional projection of hlh-1::YFP reporter expression 210 minutes after the four-cell stage (22°C; ~200 cells).

• Movie 8 -

  Movie 8. Three-dimensional projection of elt-3::YFP reporter expression 210 minutes after the four-cell stage (22°C; ~200 cells).

• Movie 9 -

  Movie 9. Three-dimensional projection of mab-21::YFP reporter expression 210 minutes after the four-cell stage (22°C; ~200 cells).

• Movie 10 -

  Movie 10. Three-dimensional projection of nhr-25::YFP reporter expression 210 minutes after the four-cell stage (22°C; ~200 cells).

• Movie 11 -

  Movie 11. Three-dimensional projection of nob-1::YFP reporter expression 210 minutes after the four-cell stage (22°C; ~200 cells).

• Movie 12 -

  Movie 12. Three-dimensional projection of tbx-8::YFP reporter expression 210 minutes after the four-cell stage (22°C; ~200 cells).

• Movie 13 -

  Movie 13. Three-dimensional projection of tbx-9::YFP reporter expression 210 minutes after the four-cell stage (22°C; ~200 cells).