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Fig. 1. Retinal astrocytes do not proliferate indefinitely in GFAP-PDGFA
mice. (A-D) Immunohistochemistry on retinal wholemounts from P7 (A,B) and P28
(C,D) animals with an anti-GFAP antibody show increased retinal astrocyte
numbers in GFAP-PDGFA transgenic mice (B,D) compared with wild-type
mice (A,C). However, retinal astrocytes do not proliferate out of control in
transgenic mice (D). (E) Retinal astrocytes in dissociated and
overnight-cultured retinae were stained with an anti-GFAP antibody and
assessed for BrdU incorporation. Retinal astrocyte proliferation ceases in
both wild-type and transgenic mice at about P8. Each data point represents the
mean±s.d. from triplicate cultures from four animals (data points
labeled with a star are significantly different at a 95% confidence level).
(F,G) In situ hybridization of retinal sections from 10-day-old mice reveals
that transgene mRNA (human PDGFA) can be readily detected in the
astrocyte layer (black arrows) in transgenic animals (F) but not in wild-type
animals (G). (H) Combined immunohistochemistry and in situ hybridization on
retinal wholemounts shows that retinal astrocytes, identified with an
anti-GFAP antibody (white staining), express PDGFRa mRNA (black
staining, white arrows) in 14-day-old wild type animals. Scale bars: 200 µm
in A-D,F,G; 20 µm in H.
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