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Fig. 2. Analysis of DjPum expression in intact and regenerating D.
japonica by in situ hybridization. Broken blue lines indicate the
blastema region, which does not contain proliferating cells. (A) Schematic
drawing of a planarian (anterior towards the top). cg, cephalic ganglia; e,
eyes; nc, nerve cords; ph, pharynx. (B-G) Dorsal view of whole-mount in situ
hybridized planarians. (B,C) Intact specimens. (D,E) Head fragments and (F,G)
lateral fragments, 3 days after transection. (B) DjPum expression.
(C) DjPum expression, 10 days after X-ray irradiation. (D)
DjPum expression. (E) Expression of DjMCM2 is shown for
comparison with the DjPum expression depicted in D. (F)
DjPum expression. (G) Expression of DjMCM2 is shown for
comparison with the DjPum expression depicted in F. (H) Schematic
drawing of a regenerating lateral fragment showing the distribution of
DjPum- and DjMCM2-positive cells (blue lines). Scale bar:
400 µm. (I) Visualization of two neoblast-like cells expressing
DjPum mRNA after in situ hybridization on dissociated cells. (L-N)
Double fluorescent in situ hybridization on dissociated cells. (L) Expression
of DjMCM2 mRNA in two neoblast-like cells. (M) DjPum mRNA
expression is detectable in one of the two cells depicted in L. (N) L and M
images were combined using Adobe Photoshop 7.0. Scale bar: 10 µm.
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