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Fig. 5. Expression of representative cell markers after DjPum RNAi in D. japonica. (A-F) DjMCM2 expression visualized by whole-mount in situ hybridization. (A) A regenerating DjPum dsRNA-injected head fragment, 15 days after the first transection. (B) A regenerating water-injected head fragment, 15 days after the first transection. (C) A DjPum dsRNA-injected head fragment lacking a visible blastema, 5 days after the second transection. (D) A regenerating water-injected head fragment, 5 days after the second transection (E) A Djeya dsRNA-injected tail fragment showing a no-eye phenotype, 5 days after the second transection. (F) A water-injected tail, 5 days after the second transection. Regenerating eyes are indicated by arrows. Scale bar: 250 µm. (G) DjMCM2, DjFGFR1, DjMHC-A, DjMHC-B, DjIFb, DjSix-1, Djops and DjClg3 expression visualized by comparative RT-PCR. RNA from DjPum dsRNA-injected planarians and water-injected controls was obtained from intact specimens 4 days after the first injection (1) and from planarian fragments sacrificed 4 days after the first (2) and the second (3) transection. DjEF2 is used as an internal amplification control.





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