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Fig. 8. FACS analysis of neoblasts in DjPum dsRNA-injected D.
japonica demonstrates a consistent reduction in the number of
proliferating cells. Graphs from a representative experiment are shown. The
cell population individuated as proliferating cells is included in the R2 box.
(A) Cells from water-injected planarians, stained with PI and analyzed for the
DNA content in the fluorescent channel FL2. (B) Analysis of cells included in
the R2 box in A, using FSC and SSC. (C) Cells from X-ray-treated planarians
sacrificed 15 days after the treatment were stained with PI and analyzed for
the DNA content in the fluorescent channel FL2. (D) Analysis of cells included
in the R2 box in C, using FSC and the SSC. (E) Cells from DjPum
dsRNA-injected planarians were stained with PI and analyzed for the DNA
content in the fluorescent channel FL2. (F) FSC and SSC analysis of cells
included in the R2 box in E, demonstrates that the cells interpreted as
proliferating cells have a similar morphology. The `nuage' at the left in A, C
and E is probably due to residual bacterial contamination. Clusters of cells
may generate the high values of fluorescence intensity observed at the right
of the R2 box in A, C and E. (G) Graphic representation of proliferating cells
(in percentage). GraphPad Prism Version 3.0 computer program was used for data
analysis. Each bar shows the mean±s.e.m. of three separate experiments.
The results were compared using the unpaired t-test;
P<0.05 was considered statistically significant.
*P<0.05; **P<0.01.
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