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Fig. 3. The cycle 10 phenotype of two cycB (A), six cycB (B), sse13m/six cycB (C, suppressor) and thr1/six cycB (D,D', enhancer) embryos. Embryos were stained with antibodies against histone (green) and phosphorylated histone H3 (red); areas stained with both appear yellow. In two cycB, six cycB and suppressor embryos, all nuclei are in interphase (green), except for polar body nuclei arrested in metaphase of cycle 1 (yellow in B,C). The enhancer (D,D') embryos have many nuclei in mitosis (yellow). Note that there are many micro/macro-nuclei and chromosomal bridges, as well as asynchronous mitoses, in thr/six cycB embryos (D'). The images are projections of ~20 optical sections with a 3-µm interval. Scale bars: in A, 50 µm; in D', 10 µm.





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