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Fig. 6. Association of LET-19 with SUR-2 and LET-425 in vivo. Nuclear extract (NE) was prepared from sur-2 mutant animals expressing only HA-tagged SUR-2 or both GFP-tagged LET-19 and HA-tagged SUR-2. Nuclear extracts and immunoprecipitation (IP) with anti-Flag (F) and anti-GFP (G) antibodies were analyzed by immunoblotting using antibodies against GFP, HA and LET-425.





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