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Fig. 1. alr-1 encodes the C. elegans homolog of Aristaless/ARX.
(A) Phylogenetic tree of the homeodomains of Aristaless/ARX family members.
The homeodomain sequences were aligned and analyzed using Bonsai 2.1
(http://calliope.gs.washington.edu/software/index.html).
The homeodomains of OTD/OTX proteins were used as outliers in this analysis.
The frequency at which a particular branch appears in 1000 bootstrap
replicates are indicated by hatchmarks: (/) – <50%; (//) –
51-80%; (///) – 81-100%. (B) Alignment of the homeodomains of ALR-1
(Ce), AL (Dm) and ARX (Mm, Hs). Identical residues
are indicated in gray. The Q50 residue is boxed. (C) (Top) alr-1 and
neighboring predicted open reading frames present on the cosmid R08B4. The
orientation of each ORF is indicated by an arrow. Sequences deleted in
alr-1(oy42) are indicated by a dashed line; inverted sequences are
indicated by a dotted line. (Bottom) Exon/intron structure of alr-1.
Sequences encoding the homeodomain are hatched. Sequences deleted in
alr-1(ok545) are indicated by a dashed line. Asterisk indicates a
point mutation in the first splice donor site in alr-1(oy56). (D)
Drosophila Al can functionally substitute for alr-1 in the
regulation of odr-7::gfp and ops-1::dsRed expression, and
for the dye-filling phenotype. The Al cDNA was expressed under the
alr-1 promoter. Adult animals grown at 25°C were examined.
n>100 for each.
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